SEM Center
The Standardized Expression Measurement (SEM) Center is now in
operation. Many of the Director's Challenge P.I.s and others wrote
letters to support the grant application that was funded to support the
SEM Center, R24 CA 91126. Your support was necessary and we appreciate
it very much.
You may access the SEM Center at www.mco.edu/medicine/semcenter The
cost of analysis is subsidized by CA 91126. As such, both the
affordability and quality of gene expression analysis at the SEM Center
are far better than through any other means. The SEM Center uses
Standardized RT (StaRT)-PCR to provide standardized, quantitative,
reproducible, sensitive gene expression measurement. StaRT-PCR reagents
for over 500 genes are now available and many more are in production.
Most of the genes related to poor adenocarcinoma outcome in the
Bhattarcharjee and Garber papers are available. The top 50 genes
described in the Beer et al paper are in production and should be
available by end of September.
All measurements made using StaRT-PCR may be entered into the same
database. This will allow a synergistic increase in knowledge.
cDNA derived from 1,000-5,000 cells (1-5 ng of RNA) is sufficient for
measurement of each gene by conventional StaRT-PCR (Crawford et al,
Molecular Diagnostics, 2001). For samples that yield very small amounts
of cDNA, multiplex StaRT-PCR (Crawford et al, BBRC, 2002) allows
measurement of at least 96 genes from the amount of cDNA derived from
1,000-5,000 cells.
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